Coding

Part:BBa_K3990003:Design

Designed by: Li Wenxi   Group: iGEM21_SMS_Shenzhen   (2021-10-01)


LaccaseCotA-386


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 885
    Illegal AgeI site found at 1192
    Illegal AgeI site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated LEU386>GLU to improve the enzyme activity.


Source

It comes from Bacillus sp.

References